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murine raw 264 7 cell line  (LGC Standards)
94
LGC Standards murine raw 264 7 cell line
Murine Raw 264 7 Cell Line, supplied by LGC Standards, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/pm41590738-308-6-25?v=LGC+Standards
Average 94 stars, based on 1 article reviews
murine raw 264 7 cell line - by Bioz Stars, 2026-07
94/100 stars
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mc38 wild type wt cell line  (Thermo Fisher)
96
Thermo Fisher mc38 wild type wt cell line
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Mc38 Wild Type Wt Cell Line, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/pmc13182474-66-13-27?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
mc38 wild type wt cell line - by Bioz Stars, 2026-07
96/100 stars
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cell line raw 264 7 gamma no  (LGC Standards)
94
LGC Standards cell line raw 264 7 gamma no
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Cell Line Raw 264 7 Gamma No, supplied by LGC Standards, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/10__7554_slash_elife__108465-650-54-68?v=LGC+Standards
Average 94 stars, based on 1 article reviews
cell line raw 264 7 gamma no - by Bioz Stars, 2026-07
94/100 stars
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water-perfused, tube-lined mitten kth4h/b  (Panasonic Healthcare)
90
Panasonic Healthcare water-perfused, tube-lined mitten kth4h/b
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Water Perfused, Tube Lined Mitten Kth4h/B, supplied by Panasonic Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/pm40581911-48-16-34?v=Panasonic+Healthcare
Average 90 stars, based on 1 article reviews
water-perfused, tube-lined mitten kth4h/b - by Bioz Stars, 2026-07
90/100 stars
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diffidence of local meteoric water line (lmwl)  (Guiyang Xintian Pharmaceutical Co Ltd)
90
Guiyang Xintian Pharmaceutical Co Ltd diffidence of local meteoric water line (lmwl)
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Diffidence Of Local Meteoric Water Line (Lmwl), supplied by Guiyang Xintian Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/pm40107141-172-8-20?v=Guiyang+Xintian+Pharmaceutical+Co+Ltd
Average 90 stars, based on 1 article reviews
diffidence of local meteoric water line (lmwl) - by Bioz Stars, 2026-07
90/100 stars
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tint-ayd® water-based dispersion line  (Elementis GmbH)
90
Elementis GmbH tint-ayd® water-based dispersion line
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Tint Ayd® Water Based Dispersion Line, supplied by Elementis GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/us12209192-44-12-14?v=Elementis+GmbH
Average 90 stars, based on 1 article reviews
tint-ayd® water-based dispersion line - by Bioz Stars, 2026-07
90/100 stars
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local meteoric water line (lmwl) for saskatoon  (Koehler Instrument)
90
Koehler Instrument local meteoric water line (lmwl) for saskatoon
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Local Meteoric Water Line (Lmwl) For Saskatoon, supplied by Koehler Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/pmc11875042-154-7-14?v=Koehler+Instrument
Average 90 stars, based on 1 article reviews
local meteoric water line (lmwl) for saskatoon - by Bioz Stars, 2026-07
90/100 stars
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temperature-controlled water bath (pro-line rp-3530  (LAUDA DR R WOBSER)
90
LAUDA DR R WOBSER temperature-controlled water bath (pro-line rp-3530
NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with <t>MC38</t> tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.
Temperature Controlled Water Bath (Pro Line Rp 3530, supplied by LAUDA DR R WOBSER, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/water+line/pmc11478857-102-25-29?v=LAUDA+DR+R+WOBSER
Average 90 stars, based on 1 article reviews
temperature-controlled water bath (pro-line rp-3530 - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with MC38 tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.

Journal: Journal for Immunotherapy of Cancer

Article Title: Synthetic DNA vaccine platform elicits potent immunity where electroporated naked-mRNA is non-immunogenic

doi: 10.1136/jitc-2025-013812

Figure Lengend Snippet: NCV delivered by hpDNA-EP is as effective as equimolar pDNA-EP. ( A ) Experimental design. C57BL/6 female mice received three immunizations with M20-hpDNA-EP (6.7–3.35 and 1.68 µg) or M20-pDNA-EP (10–5 and 2.5 µg) on days 0, 21 and 42, in a prophylactic set-up. On day 49, immune responses were measured in PBMCs. 1 month after the last immunization, mice were inoculated with MC38 tumor cells s.c. in the right flank (n=5) and the tumor size was monitored. Unvaccinated naïve mice (n=5) were also challenged with the tumor cell line. ( B ) Percentages of IFN-γ + and TNF-α + CD4 + and CD8 + T cells measured in PBMCs 1 week after the last vaccination are shown. PBMCs were restimulated with the peptide pool identified in the IFN-γ-ELISpot assay. ( C–D ) Tumor volumes and survival determined over time are shown. ( E ) Second tumor challenge on day 160. M20-hpDNA-EP vaccinated mice (dose 6.7 µg) surviving the first tumor challenge were re-challenged on the opposite flank. A naïve control group of mice (untreated) was challenged in parallel (n=5). The experiment shown is one out of three performed. The Mann-Whitney test and log-rank (Mantel-Cox) test were used to make comparisons: *p<0.05; **p<0.01; ***p<0.001 versus untreated group. ELISpot, enzyme-linked immunospot; FC, Flow Cytometry; hpDNA, hairpin DNA; IFN, interferon; NCV, neoantigen cancer vaccine; PBMCs, peripheral blood mononuclear cells; pDNA, plasmid DNA; s.c., subcutaneous; TNF, tumor necrosis factor.

Article Snippet: A monoclonal population of MC-38 Luciferase (Luc) cell line was established by infecting MC38 wild-type (WT) cell line with lentivirus containing luciferase gene and selected with puromycin (Invitrogen cat. J67236.XF).

Techniques: Enzyme-linked Immunospot, Control, MANN-WHITNEY, Flow Cytometry, Plasmid Preparation

M20-hpDNA-EP synergizes with αCTLA-4 in a therapeutic protocol and protects mice from tumor metastasis. ( A ) Experimental design: C57BL/6 mice (n=5) were inoculated with MC38 tumor cells s.c. in the right flank and treated with αCTLA-4 and vaccine as depicted. ( B ) Subcutaneous tumor growth under therapeutic setting with or without adjuvants treatment. ( C ) Survival curve under therapeutic setting with or without adjuvants treatment. ( D ) Representative image of CD8 + IFN-γ + cells in tumor and spleen. ( E ) Graph of CD8 + IFN-γ + cells in tumor and spleen. ( F ) Vaccination scheme for lung metastasis experiment. ( G ) Representative image of lung metastasis with India ink staining. ( H ) Graph of lung metastatic area, n=6–9 animals per group. ( I ) Survival curve comparison between untreated mice and M20-hpDNA-EP vaccinated mice, n=5 animals per group. Data are represented as mean±SEM. Statistical analysis was performed with one-way ANOVA in B-E-H and log-rank (Mantel-Cox) test in C-I. *p<0.05, **p<0.001; ***p<0.0001, when not specified is not significant. ANOVA, analysis of variance; CTLA-4, cytotoxic T-lymphocyte associated protein 4; EP, electroporation; FC, Flow Cytometry; hpDNA, hairpin DNA; ICI, immune checkpoint inhibitor; IFN, interferon; PD-L1, programmed death-ligand 1.

Journal: Journal for Immunotherapy of Cancer

Article Title: Synthetic DNA vaccine platform elicits potent immunity where electroporated naked-mRNA is non-immunogenic

doi: 10.1136/jitc-2025-013812

Figure Lengend Snippet: M20-hpDNA-EP synergizes with αCTLA-4 in a therapeutic protocol and protects mice from tumor metastasis. ( A ) Experimental design: C57BL/6 mice (n=5) were inoculated with MC38 tumor cells s.c. in the right flank and treated with αCTLA-4 and vaccine as depicted. ( B ) Subcutaneous tumor growth under therapeutic setting with or without adjuvants treatment. ( C ) Survival curve under therapeutic setting with or without adjuvants treatment. ( D ) Representative image of CD8 + IFN-γ + cells in tumor and spleen. ( E ) Graph of CD8 + IFN-γ + cells in tumor and spleen. ( F ) Vaccination scheme for lung metastasis experiment. ( G ) Representative image of lung metastasis with India ink staining. ( H ) Graph of lung metastatic area, n=6–9 animals per group. ( I ) Survival curve comparison between untreated mice and M20-hpDNA-EP vaccinated mice, n=5 animals per group. Data are represented as mean±SEM. Statistical analysis was performed with one-way ANOVA in B-E-H and log-rank (Mantel-Cox) test in C-I. *p<0.05, **p<0.001; ***p<0.0001, when not specified is not significant. ANOVA, analysis of variance; CTLA-4, cytotoxic T-lymphocyte associated protein 4; EP, electroporation; FC, Flow Cytometry; hpDNA, hairpin DNA; ICI, immune checkpoint inhibitor; IFN, interferon; PD-L1, programmed death-ligand 1.

Article Snippet: A monoclonal population of MC-38 Luciferase (Luc) cell line was established by infecting MC38 wild-type (WT) cell line with lentivirus containing luciferase gene and selected with puromycin (Invitrogen cat. J67236.XF).

Techniques: Staining, Comparison, Electroporation, Flow Cytometry